Regulation of Twist and BMP Antagonists Expression in Human Periodontal Ligament Cells

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  • 培養ヒト歯根膜細胞におけるTwistと各種BMP antagonist の発現制御
  • バイヨウ ヒト シコンマク サイボウ ニ オケル Twist ト カクシュ BMP antagonist ノ ハツゲン セイギョ

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Twist and bone morphogenetic protein (BMP) antagonists may play an important role in maintenance of constancy of periodontal ligament (PL), by which negatively regulate osteoblastic differentiation of cells existing in PL. In the present study, changes of these molecules expression by inducing osteoblastic differentiation were assessed in human PL (HPL) cells. We further investigated regulatory effects of BMP antagonists on osteoblastic differentiation in the cells. When HPL cells were cultured in osteogenic differentiation medium (ODM), the cells were differentiated into mature osteoblast-like cells, and endogenous BMP2/ 4 mediated the ODM-induced ostoblastic differentiation. HPL cells constitutively expressed twist as well as BMP antagonists; gremlin and follistatin at mRNA and protein levels, whereas, expression of chordin could not be detected at a protein level. Twist expression was increased in cells cultured with ODM and in BMP-2-treated cells. Both of gremlin and follistatin were stimulated to express in cells cultured in ODM, whereas BMP-2 had no effect on those expression. In addition, each of recombinant gremlin and recombinant follistatin significantly suppressed the ODM-induced osteoblastic differentiation. These results suggest that under physiological condition, twist, gremlin and follistatin, which are constitutively expressed in HPL cells, negatively regulate osteoblastic differentiation of the cells. Further, the induction of osteoblastic differentiation in HPL cells simultaneously enhances twist expression, while it suppresses expression of gremlin and follistatin, as the result, the osteoblastic differentiation may be modulated

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