An Electrophoretic Analysis of the Hemoglobin of Japanese Toads

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  • 電気泳動法による日本産ヒキガエルのヘモグロビン分析

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An electrophoretic analysis of the hemoglobin of Japanese toads, Bufo bufo japonicus, B. b, formosus, and B. torrenticola was made from the taxonomic point of view. B. torrenticola had one hemoglobin component, irrespective of age and sex, whereas B. b. formosus had two components, one of which is identical with that of B. torrenticola. The banding pattern of B. b. japonicus, showing either one or two hemoglobin components, is identical either with B. torrenticola or B. b. formosus, respectively. This variation in B. b. japonicus seems to indicate the existence of polymorphism within a population. Systematic relationships between these forms and European B. b, bufo were examined in the light of hemoglobin components and other non-biochemical evidences, and it was concluded that the Japanese group have diverged considerably from the European species.

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