Suppression of .ALPHA.-amylase gene expression by antisense oligodeoxynucleotide in barley cultured aleurone layers.

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  • TSUTSUMI Nobuhiko
    Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
  • KANAYAMA Keiko
    Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
  • TANO Shigemitsu
    Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo

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  • アンチセンスオリゴヌクレオチドによるオオムギアリューロン層におけるα-アミラーゼ遺伝子発現の抑制〔英文〕
  • アンチセンス オリゴヌクレオチド ニ ヨル オオムギ アリューロンソウ ニ オケ

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Abstract

Antisense oligodeoxynucleotides (ODNs) have been applied to regulate gene expression using cell-free media or animal cells. Here we demonstrate the specific inhibition of barley α-amylase gene expression by synthetic antisense ODNs. In a cell free system using wheat-germ extracts, 5 μM of a 20-mer antisense ODN prevented the synthesis of the polypeptide corresponding to the predetermined length of α-amylase translated in vitro, whereas there was no effect on other protein synthesis. Furthermore, in cultured aleurone cells, a-amylase activity was efficiently decreased by addition of ODNs. At the concentrations higher than 5 μM, antisense ODN inhibited α-amylase gene expression almost completely. These results imply that ODN could transport into the cultured aleurone cells crossing the cell membrane, and regulate specific gene expression. This simple model system could be applicable not only for the analysis of the α-amylase multigene family in barley but also for studying functions of cryptic genes in higher plant.<br>

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