Suppression of .ALPHA.-amylase gene expression by antisense oligodeoxynucleotide in barley cultured aleurone layers.
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- TSUTSUMI Nobuhiko
- Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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- KANAYAMA Keiko
- Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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- TANO Shigemitsu
- Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
Bibliographic Information
- Other Title
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- アンチセンスオリゴヌクレオチドによるオオムギアリューロン層におけるα-アミラーゼ遺伝子発現の抑制〔英文〕
- アンチセンス オリゴヌクレオチド ニ ヨル オオムギ アリューロンソウ ニ オケ
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Description
Antisense oligodeoxynucleotides (ODNs) have been applied to regulate gene expression using cell-free media or animal cells. Here we demonstrate the specific inhibition of barley α-amylase gene expression by synthetic antisense ODNs. In a cell free system using wheat-germ extracts, 5 μM of a 20-mer antisense ODN prevented the synthesis of the polypeptide corresponding to the predetermined length of α-amylase translated in vitro, whereas there was no effect on other protein synthesis. Furthermore, in cultured aleurone cells, a-amylase activity was efficiently decreased by addition of ODNs. At the concentrations higher than 5 μM, antisense ODN inhibited α-amylase gene expression almost completely. These results imply that ODN could transport into the cultured aleurone cells crossing the cell membrane, and regulate specific gene expression. This simple model system could be applicable not only for the analysis of the α-amylase multigene family in barley but also for studying functions of cryptic genes in higher plant.<br>
Journal
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- The Japanese Journal of Genetics
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The Japanese Journal of Genetics 67 (2), 147-154, 1992
The Genetics Society of Japan
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Details 詳細情報について
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- CRID
- 1390001205443731328
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- NII Article ID
- 10006699135
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- NII Book ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
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- NDL BIB ID
- 3773793
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- PubMed
- 1524833
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed