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- YAKURA Kimitaka
- Department of Biology, Faculty of Education, Kanazawa University
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- KATO Atsushi
- Department of Botany, Faculty of Science, Hokkaido University
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- TANIFUJI Shigeyuki
- Department of Botany, Faculty of Science, Hokkaido University
書誌事項
- タイトル別名
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- Cytological localization of highly repeated DNA sequences, the FokI sequence family and BamHI sequence families, in Vica faba chromosomes.
- ソラマメ ノ コウド ハンプク DNA ハイレツ FokI オヨビ BamHI
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抄録
Cytological localization of one of the highly repeated DNA sequences of Vicia faba, the FokI repeat sequence family, was determined by in situ hybridization. This sequence family was described in our previous paper (Kato et al. 1984) as tandem arrays of 59bp unit sequences. The distribution pattern of silver grains showed that the FokI family sequences are exclusively localized in certain median regions in the long arms of all five pairs of the subtelocentric (S) chromosomes, but are not found in any portions in the metacentric (M) chromosomes. These median regions in the S long arms correspond closely to the positions of C-band heterochromatin revealed by preferential Giemsa staining. Since M chromosomes also possess C-bands, the absence of the FokI repeat sequences in the M chromosomes indicates that V. faba C-band heterochromatin consists of at least two different DNA sequences. V. faba chromosomes also contain other repetitive sequences, which have been characterized as the 250, 850, 900, 990, 1150, 1500 and 1750 by BamHI sequence families (Kato et al. 1985). In situ hybridization with nicktranslated 250 and 1500bp BamHI family sequences indicated that these repeated sequences are generally spread all over the chromosomes.
収録刊行物
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- 遺伝学雑誌
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遺伝学雑誌 62 (4), 325-332, 1987
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205443830784
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- NII論文ID
- 130000060716
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- NII書誌ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
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- NDL書誌ID
- 3158540
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可