Insertional mutagenesis in Drosophila II. P element mediated transformation of Drosophila yakuba.

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  • TOGASHI Shin
    Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
  • UEDA Ryu
    Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
  • TAKAHISA Manabu
    Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
  • MIKUNI Misa
    Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
  • KONDO Kanae
    Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
  • MIYAKE Tadashi
    Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11

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  • ショウジョウバエにおける挿入突然変異誘発-2-PエレメントによるDrosophila yakubaの形質転換〔英文〕
  • ショウジョウバエ ニ オケル ソウニュウ トツゼン ヘンイ ユウハツ 2 Pエ

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Abstract

Drosophila yakuba, a member of melanogaster subgroup being free of P element, acquired resistance to an antibiotic neomycin by the transformation utilizing P element. In this species, the transformation frequency was comparable to that of D. melanogaster. Further, the occurrence of 8 base pairs duplication upon the insertion of the element was confirmed. These facts suggest that the P element could be inserted into the genome in the same manner, even in D. yakuba. Any consensus for preferential insertion could not be found on the nucleotide sequence as in D. melanogaster. However, it is noticeable that a series of the short palindromic stretches was common around the insertion sites in both species. It suggests that a structural feature of DNA plays a role as a landmark for P element insertion.<br>

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