Insertional mutagenesis in Drosophila II. P element mediated transformation of Drosophila yakuba.
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- TOGASHI Shin
- Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
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- UEDA Ryu
- Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
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- TAKAHISA Manabu
- Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
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- MIKUNI Misa
- Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
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- KONDO Kanae
- Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
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- MIYAKE Tadashi
- Laboratory of Cell Biology Mitsubishi Kasei Institute of Life Sciences 11
Bibliographic Information
- Other Title
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- ショウジョウバエにおける挿入突然変異誘発-2-PエレメントによるDrosophila yakubaの形質転換〔英文〕
- ショウジョウバエ ニ オケル ソウニュウ トツゼン ヘンイ ユウハツ 2 Pエ
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Description
Drosophila yakuba, a member of melanogaster subgroup being free of P element, acquired resistance to an antibiotic neomycin by the transformation utilizing P element. In this species, the transformation frequency was comparable to that of D. melanogaster. Further, the occurrence of 8 base pairs duplication upon the insertion of the element was confirmed. These facts suggest that the P element could be inserted into the genome in the same manner, even in D. yakuba. Any consensus for preferential insertion could not be found on the nucleotide sequence as in D. melanogaster. However, it is noticeable that a series of the short palindromic stretches was common around the insertion sites in both species. It suggests that a structural feature of DNA plays a role as a landmark for P element insertion.<br>
Journal
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- The Japanese Journal of Genetics
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The Japanese Journal of Genetics 67 (4), 291-297, 1992
The Genetics Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001205443999232
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- NII Article ID
- 10006699434
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- NII Book ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
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- NDL BIB ID
- 3813080
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- PubMed
- 1338284
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed