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- ISHIWA Hiromi
- Yakult Institute for Microbiological Research
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- SHIBAHARA Harue
- Yakult Institute for Microbiological Research
書誌事項
- タイトル別名
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- New shuttle vectors for Escherichia coli and Bacillus subtilis. II. Plasmid pHY300PLK, a multipurpose cloning vector with a polylinker, derived from pHY460.
- ダイチョウキン ト コソウキン デ キョウヨウデキル アタラシイ シャトル ベ
- II. Plasmid pHY300PLK, a multipurpose cloning vector with a polylinker, derived from pHY460
この論文をさがす
抄録
In vivo and in vitro recombination techniques were used to construct a new cloning vector, pHY300PLK (4.7 kb) from the shuttle vector pHY460 (7kb). The newly derived shuttle vector can replicate and express the tetracycline resistance gene (TcR) in both Escherichia coli and Bacillus subtilis. pHY300PLK contains the TcR gene, the ampicillin resistance gene (ApR), two replication origins for E. coli and B. subtilis and a polylinker derived from πAN7. The unique cloning sites are BalI, BamHI, BanI, BglI, BglII, BstEII, EcoRI, EcoRV, HindIII, HpaI, SalI, SmaI, PvuI and XbaI, pHY300 PLK is characterized as a copy-number mutant in E. coli. (Key words: shuttle vector; B, subtilis; tetracycline; ampicillin; copy-number mutant; incompatibility)
収録刊行物
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- 遺伝学雑誌
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遺伝学雑誌 60 (3), 235-243, 1985
日本遺伝学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390001205444379136
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- NII論文ID
- 130000060539
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- NII書誌ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
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- NDL書誌ID
- 3046627
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可