Karyotyping of the chromosomes and physical mapping of the 5S rRNA and 18S-26S rRNA gene families in five different species in Capsicum.
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- Youn-Kyu Park
- Department of Agronomy, Kangwon National University
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- Kim Byung-Dong
- Department of Horticulture, Seoul National University
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- Kim Byung-Soo
- Department of Horticulture, Kyungbook National University
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- Armstrong Kenneth C.
- Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada
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- Kim Nam-Soo
- Department of Agronomy, Kangwon National University
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Description
Since clear identification of individual chromosomes in Capsicum species was not possible due to the gradient in sizes and similar morphology, in situ hybridization using 5S rDNA and 18S-26S rDNA probes was utilized to karyotype the chromosomes of the five species, Capsicum annuum, C. chinense, C. frutescens, C. baccatum, and C. pubescens. The 5S rDNA located on only one pair of chromosomes in all five species. The 5S rDNA carrying chromosome was designated as chromosome 1 by the synteny relationship with tomato. In two species, C. baccatum and C. pubescens, the 5S rDNA was physically linked with a 18S-26S rDNA locus. The number and chromosomal location of 18S-26S rDNA loci were highly variable among the species. The number of loci was as few as two in C. annuum and as many as 14 in C. baccatum. The chromosomal positions of the 18S-26S loci were in the telomere regions in the corresponding chromosomes and some chromosomes showed hybridization signals in this region of both long and short arms. A pair of acrocentric chromosomes carrying the 18S-26S rDNA locus in the satellite region was found in all five species. The in situ and Southern hybridization patterns of the rDNAs were similar between C. annuum and C. chinense. C. frutescens was similar to C. chinense. C. baccatum and C. pubescens were speculated to be less related to the former three Capsicum species. The variable nature of the 18S-26S rDNA loci in the Capsicum species might provide a good model for rDNA evolution.<br>
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 74 (4), 149-157, 1999
The Genetics Society of Japan
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Details 詳細情報について
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- CRID
- 1390001205444564096
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- NII Article ID
- 10004718101
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- NII Book ID
- AA11077421
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- COI
- 1:CAS:528:DC%2BD3cXos1GlsA%3D%3D
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- ISSN
- 18805779
- 13417568
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- NDL BIB ID
- 4922672
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed