DNA fingerprinting of animal genomes by CA-repeat primed polymerase chain reaction.

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  • ISHIBASHI Yasuyuki
    Chromosome Research Unit, Faculty of Science
  • ABE Syuiti
    Chromosome Research Unit, Faculty of Science Laboratory of Cytogenetics, Graduate School of Environmental Earth Science, Hokkaido University
  • YOSHIDA Michihiro C.
    Chromosome Research Unit, Faculty of Science Laboratory of Cytogenetics, Graduate School of Environmental Earth Science, Hokkaido University

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Other Title
  • 動物ゲノムにおけるCA繰返し配列プライマーを用いたDNA fingerprint法
  • 動物ゲノムにおけるCA繰返し配列プライマーを用いたDNA fingerprint法〔英文〕
  • ドウブツ ゲノム ニ オケル CA クリカエシ ハイレツ プライマー オ モチ

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CA-repeat primed polymerase chain reaction (CAP-PCR), using degenerate primers which anneal at the ends of (CA)n sequences in eukaryotic gejiomes, was attempted to assess its potential to monitor the genomic polymorphisms in various animals. Three mammalian, three avian, one fish and one insect species were examined and all showed primer-specific DNA fingerprints by CAP-PCR. Polymorphic bands observed in a laboratory-bred vole family were segregated in Mendelian manner. The present CAP-PCR DNA fingerprinting therefore is a simple and useful method for examining genomic variations in most animals without prerequisite knowledge of DNA sequences.<br>

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