Phenotypic and molecular characterization of croaker, a new mating behavior mutant of Drosophila melanogaster.
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- YOKOKURA Takakazu
- Mitsubishi Kasei Institute of Life Sciences Department of Science and Technology, Waseda University
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- UEDA Ryu
- Mitsubishi Kasei Institute of Life Sciences
Bibliographic Information
- Other Title
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- キイロショウジュバエの新しい配偶行動異常変異体croakerの表現型と分子的解析〔英文〕
- キイロショウジュバエ ノ アタラシイ ハイグウ コウドウ イジョウ ヘンイタイ
- Phenotypic and molecular characterization of <italic>croaker</italic>, a new mating behavior mutant of <italic>Drosophila melanogaster</italic>
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Abstract
Mating of Drosophila melanogaster is a stereotypically patterned behavior consisting of a fixed sequence of actions that is primarily under genetic control. Although courtship can be easily monitored and quantified, little is known about its neural basis. To obtain a better understanding of cellular and molecular mechanisms underlying courtship, we have isolated mutants that disrupt specific aspects of mating behavior. The croaker mutant was isolated from approximately 1,000 lines harboring single P-element insertions by screening for aberrant courtship song: croaker males often generate polycyclic pulse song while most of the song pulses are monocyclic in the wild-type. The mutant is also defective in flight. Intracellular recordings of excitatory junction potentials from larval body wall muscles and Ca++ action potentials from adult indirect flight muscles demonstrated that neuromuscular transmission and Ca++ electrogenesis in the muscle fibers are not impaired by the croaker mutation. To define the croaker gene molecularly, genomic DNA surrounding the P-element insertion site was cloned by plasmid rescue and subsequent screening of a cosmid library. Northern blotting with the genomic DNA probes detected three transcripts in the wild-type, which were not expressed in the croaker mutant.<br>
Journal
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- The Japanese Journal of Genetics
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The Japanese Journal of Genetics 70 (1), 103-117, 1995
The Genetics Society of Japan
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Details 詳細情報について
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- CRID
- 1390001205445015936
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- NII Article ID
- 10002506989
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- NII Book ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
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- NDL BIB ID
- 3602654
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- PubMed
- 7772376
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed