Detection of H-2K mRNA in mouse 8-cell embryo by cDNA cloning.

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  • NAGATA Toshi
    Department of Microbial Genetics, Research Institute for Microbial Diseases, Osaka University
  • NOZAKI Masami
    Department of Microbial Genetics, Research Institute for Microbial Diseases, Osaka University
  • MORITA Takashi
    Department of Microbial Genetics, Research Institute for Microbial Diseases, Osaka University
  • MATSUSHIRO Aizo
    Department of Microbial Genetics, Research Institute for Microbial Diseases, Osaka University

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  • cDNAクローニングによる8細胞期マウス胚におけるH-2K mRNAの検出〔英文〕
  • c DNA クローニング ニ ヨル 8 サイボウキ マウス ハイ ニ オケル

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Mouse MHC class I gene expression in 8-cell embryo was examined by cDNA cloning. We constructed a cDNA library from 8-cell embryos of ICR mice and isolated a class I cDNA from 3.0×105 phage clones of the library. Sequencing analysis of this clone revealed it to include the cDNA fragment extending from the exon 6 of the cytoplasmic portion to 3′ untranslated region 1 of H-2K gene. Qa, Tla or other embryonic class I cDNA have not been isolated in the library.

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