大腸菌dnaK変異種およびdnaJ変異種におけるRNA合成阻害の解除について

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  • TANAKA Masashi
    Department of Biology, Faculty of Science, Tokyo Metropolitan University
  • WADA Morimasa
    Department of Biology, Faculty of Science, Tokyo Metropolitan University
  • ITIKAWA Hiraku
    Department of Biology, Faculty of Science, Tokyo Metropolitan University

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タイトル別名
  • Abortive relaxation of RNA synthesis for cellular DNA replication affected by dnaK(Ts) and dnaJ(Ts) mutations in Escherichia coli K-12.
  • 大腸菌dnaK変異種およびdnaJ変異種におけるRNA合成阻害の解除について〔英文〕
  • ダイチョウキンdnaK ヘンイシュ オヨビ dnaJ ヘンイシュ ニ オケル

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Cellular DNA and RNA syntheses in the strains carrying a mutation in relA gene in combination with a mutation in either dnaK or dnaJ gene were examined. RNA synthesis was relaxed in either combination both in the presence and in the absence of an exess amount of L-valine. However, DNA syntheses remained inhibited under the same conditions, suggesting that the thermosensitive DNA synthesis and RNA synthesis which are coexhibited in the dnaK(Ts) and dnaJ(Ts) mutants are not directly related each other. Thus the relaxed RNA synthesis in the double mutants did not reflect on the restoration of their thermoresistant DNA synthesis.

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