Inhibitory Effect of Low-Power Diode Laser on Neurite Elongation of Cultured Trigeminal Ganglion

DOI 2 Citations
  • CHEN Ming-yu
    Dept. of Oral and Maxillofacial Surgery, School of Medicine, Kobe University.
  • FUJITA Kunio
    Dept. of Oral and Maxillofacial Surgery, School of Medicine, Kobe University.
  • ISHII Junnosuke
    Dept. of Oral and Maxillofacial Surgery, School of Medicine, Kobe University.
  • SHIMADA Keikiti
    Dept. of Oral and Maxillofacial Surgery, School of Medicine, Kobe University.
  • HIRATA Tatumi
    Laboratory of Neurosience. Department of Molecular Biology, School.
  • FUJISAWA Hajime
    Laboratory of Neurosience. Department of Molecular Biology, School.

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  • 培養三叉神経線維に及ぼす低出力半導体レーザーの抑制効果

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Abstract

Ga-Al-As diode laser system has recently been used for pain alleviation therapy. The mechanism of the pain attenuation, however, has not yet been clarified. We tried laser irradiation on cultured trigeminal ganglion and investigated the growth of neurite. Trigeminal ganglia were prepared from Xenopus laevis at stages 50-53 and cultured for 5 days. Gontinuous wave of Ca-Al-As diode laser was irradiated on the ganglion for 30 minutes, with wave length of 830nm and output power of 20mW. Immediately after irradiation, growth cornes of neurites shrinked and stopped elongation. Some of neurites degenerated. Seven hours later, neurites began to elongate again. Similar results were obtained in single neuron culture experiments. Neurites of laser irradiation groups elongated at an average rate of -2.37μm per 3 hours. In control groups, neurites grew at 39.82μm per 3 hours. This inhibitory effect on neurite elongation may relate to the pain attenuation mechanism of low power laser irradiation.

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