Electron Microscopic Study of Dengue-4 Virus

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  • Electron Microscopic Study of Dengue 4

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The growth and development of dengue-4 virus in mice and Vero cells were studied morphologically by electron microscopy, fluorescent antibody staining, and acid phosphatase reaction, and the following results were obtained.<BR>Before the manifestation of virus reproduction, pronounced acid phosphatase activity of host cells was observed. At this stage, specific fluorescence against dengue-4 virus appeared in the perinuclear region of infected Vero cells and gradually increased. By electron microscopy, virus particles were first found in the cytoplasm or in the endoplasmic reticula after a latent period of 18 hours, and their number increased successively. The virus particles then assembled in the vesicles or intracellular space, usually in random arrangement. In the late period of infection, cells became degenerated and massively arranged virus particles were found more easily both intra- and intercellularly. These findings corresponded well with the results of infectivity assay, the cytopathic effects observed, and the paralysis and death of mice. A budding process through cellular membrane structures for dengue-4 virus maturation was suggested.<BR>The dengue-4 virus particles were found to be spherical in shape and estimated to be approximately 45-50 nm in diameter. Each was composed of an outer membrane of 4 nm thickness and an electrondense core,25 nm in diameter. Incomplete particles were not observed. Immature virus particles were also observed 24 hours after inoculation.

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