Quantitative Studies on the Rate of Cell Production in the Thymolymphatic Organs I. A Simple Method for Determination of the Mitotic Index in Suspension of Cell Nuclei
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- Ito Hirohiko
- Department of Anatomy, Yamaguchi Medical School, Ube
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説明
A simple method for dete r m ination of the mitotic index in suspension of cell nuclei prepared from lymphoid organs has been described. This method tends to yield higher values than a similar method previously described by Christensen (1950). The recommended procedure is as follows: the lymphoid organ to be examined is fixed in N/4 HCl for 2-3 hours to prepare a suspension of nuclei from the whole organ. After staining the nuclei with Mayer's acid hemalum, film preparations on slides are made from the suspension. The mitotic index is then determined after counting 5,000nuclei from each film. It is essential to make sufficiently thin films from the suspension, so that cells in mitosis can be counted with accuracy.
収録刊行物
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- Okajimas Folia Anatomica Japonica
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Okajimas Folia Anatomica Japonica 33 (5-6), 275-283, 1959
Editorial Board of Okajimas Folia Anatomica Japonica
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詳細情報 詳細情報について
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- CRID
- 1390001205460759296
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- NII論文ID
- 130004990273
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- ISSN
- 0030154X
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可