Fine Structural Localization of Non-specific Alkaline Phosphatase Activity in the Small Intestine of the Rat; an Intravital Perfusion Experiment with Azo Dye Method

DOI Web Site

この論文をさがす

抄録

Non-specific alkaline phosphatase activity was electron microscopically investigated in the small intestine of the rat. A mixture of 1-naphthyl phosphate and hexazotized pararosanilin was intravascularly or -luminally perfused into the small intestine of living animals. About 3 minutes after intraluminal or -vascular perfusion, reaction products of a reddish purple colour appeared on the luminal surface of the small intestine; intense reaction was detected on the epithelium of the duodenum and jejunum, whereas the terminal portion of the ileum seems to be devoid of the activity of this enzyme.<BR>In the electron microscope, electron dense reaction p r oducts (about 40 mμ in diameter) were observed on the membranes covering microvilli of the intestinal epithelium. When the incubating mixture was intravascularly perfused into the small intestine, reaction products were seen not only on the plasma membrane of microvilli but also on the lateral cell membrane of the duodenal and jejunal epithelium.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ