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- Kondo Shu
- Invertebrate Genetics Laboratory, National Institute of Genetics Department of Genetics, the Graduate University for Advanced Studies
書誌事項
- タイトル別名
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- New horizons in genome engineering of <i>Drosophila melanogaster</i>
この論文をさがす
説明
Drosophila melanogaster has the longest history as a genetic model system and even in the present day remains the front runner in diverse fields of biology. However, lack of a convenient method to make specified modifications to endogenous genes has been a pain in the neck for many fly geneticists for decades. Synthetic nuclease technologies, especially the CRISPR/Cas9 system, hold great promise for a breakthrough. Synthetic nucleases are programmable nucleases that can be directed to cleave a specified sequence in the genome. Deleterious mutations can be efficiently induced by expression of a synthetic nuclease that targets a gene of interest. Precise modification of the target site, such as a reporter gene knock-in, is also possible by simultaneous delivery of a synthetic nuclease and a targeting vector. Here I summarize recent advances in synthetic nuclease technologies and discuss their possible applications to Drosophila genetics.
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 89 (1), 3-8, 2014
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205472499584
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- NII論文ID
- 130004137820
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- NII書誌ID
- AA11077421
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- DOI
- 10.1266/ggs.89.3
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- COI
- 1:STN:280:DC%2BC2cjhvVGnsw%3D%3D
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 025424906
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- PubMed
- 24817756
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可