Sae2p phosphorylation is crucial for cooperation with Mre11p for resection of DNA double-strand break ends during meiotic recombination in Saccharomyces cerevisiae
-
- Terasawa Masahiro
- Iwate College of Nursing
-
- Ogawa Tomoko
- Iwate College of Nursing
-
- Tsukamoto Yasumasa
- Iwate College of Nursing
-
- Ogawa Hideyuki
- Iwate College of Nursing
この論文をさがす
説明
Meiotic recombination is initiated by the introduction of DNA double-strand breaks (DSBs) at recombination hotspots. DSB ends are resected to yield ssDNA, which is used in a homology search. Sae2p, which is involved in the resection of DSB ends, is phosphorylated by the Mec1p and Tel1p kinases during meiosis. To clarify the role of Sae2p phosphorylation in meiotic recombination, three mutants with alanine substitutions (at two putative Mec1/Tel1 phosphorylation sites near the N terminus, at three sites near the C terminus or at all five sites) were constructed. Analysis of DSB ends during meiotic recombination demonstrated that phosphorylation of the three C-terminal phosphorylation sites is necessary for DSB end resection and that phosphorylation of the two N-terminal phosphorylation sites is required for the efficient initiation of DSB end resection. Sae2p was localized on meiotic chromosomes in the rad50S and mre11-H125R mutants, which accumulate DSB ends. Alanine substitutions of all phosphorylation sites did not affect localization of Sae2p on meiotic chromosomes. Although colocalization of Sae2p with Mre11p and recombinant formation were observed in the N-terminally mutated and the C-terminally mutated strains, these processes were drastically impaired in the quintuple mutant. These results indicate that phosphorylation of Sae2p is required to initiate resection and to improve the efficiency of resection through cooperation with the Mre11-Rad50-Xrs2 complex.<br>
収録刊行物
-
- Genes & Genetic Systems
-
Genes & Genetic Systems 83 (3), 209-217, 2008
日本遺伝学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205472833024
-
- NII論文ID
- 10024396114
-
- NII書誌ID
- AA11077421
-
- COI
- 1:STN:280:DC%2BD1cvovF2muw%3D%3D
-
- ISSN
- 18805779
- 13417568
-
- NDL書誌ID
- 9559817
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可