Identification of pTi-SAKURA DNA region conferring enhancement of plasmid incompatibility and stability
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- Yamamoto Shinji
- Department of Biological Science, Graduate School of Science, Hiroshima University
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- Uraji Misugi
- Department of Biological Science, Graduate School of Science, Hiroshima University
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- Tanaka Katsuyuki
- Department of Biological Science, Graduate School of Science, Hiroshima University
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- Moriguchi Kazuki
- Department of Biological Science, Graduate School of Science, Hiroshima University
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- Suzuki Katsunori
- Department of Biological Science, Graduate School of Science, Hiroshima University
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説明
In Agrobacterium tumefaciens, the stability of Ti plasmids differs depending on the strain. So far, little is known about genes that cause the difference in stability. The repABC operon is responsible for replication and incompatibility of Ti plasmids. We constructed recombinant plasmids carrying the repABC operon and different portions of pTi-SAKURA. Cells having the recombinant plasmids that harbored a 2.6-kbp NheI fragment of pTi-SAKURA were found to be transformed via conjugation 100-fold less frequently with a small incompatible repABC plasmid than cells having the recombinant plasmids lacking the 2.6-kbp NheI fragment. Since the phenomenon occurred only when the resident and incoming plasmids belonged to the same incompatibility group, it was suggested that the 2.6-kbp NheI fragment bears the potential enhancing incompatibility. The fragment contained an operon consisting of two open reading frames, tiorf24 and tiorf25. tiorf24 is an orphan gene, whereas tiorf25 is a homologue of a group of plasmid stability genes. Removal of the 2.6-kbp fragment from the resident pTi-SAKURA increased the resident plasmid ejection ratio by the incoming repABC plasmid, whereas addition of the fragment to pTiC58 decreased the ejection ratio, and the loss ratio during growth at 37°C. These data suggest that tiorf24 and tiorf25 are responsible for the stability of pTi-SAKURA, and reduce, in the host bacterium, the frequency of ejection of the resident plasmid, presumably through an incompatibility mechanism.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 82 (3), 197-206, 2007
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205473294464
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- NII論文ID
- 10024394297
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 8821654
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可