Cytogenetic characterization of the bay scallop, Argopecten irradians irradians, by multiple staining techniques and fluorescence in situ hybridization
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- Huang Xiaoting
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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- Hu Jingjie
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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- Hu Xiaoli
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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- Zhang Can
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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- Zhang Lingling
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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- Wang Shi
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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- Lu Wei
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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- Bao Zhenmin
- Laboratory of Marine Genetics and Breeding (MGB), Division of the Life Science and Technology, Ocean University of China
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説明
The chromosomes of Argopecten irradians irradians were studied by various cytogenetic approaches. Conventional chromosome characterization built on C-banding, DAPI-staining, and silver staining was complemented by the physical mapping of ribosomal DNA and telomeric sequence (TTAGGG)n by FISH. Results showed that the constitutive heterochromatin revealed by C-banding was mainly distributed at telomeric and centromeric regions. However, interstitial C-bands were also observed. The pattern of DAPI banding was almost consistent with that of C-banding. Silver staining revealed that NORs were located on the short arms of chromosome 3 and 10, and this was further confirmed by FISH using 18S-28S rDNA. 5S rDNA was mapped as two distinguishable loci on the long arm of chromosome 11. 18S-28S and 5S rDNA were located on different chromosomes by sequential FISH. FISH also showed that the vertebrate telomeric sequence (TTAGGG)n was located on both ends of each chromosome and no interstitial signals were detected. Sequential 18S-28S rDNA and (TTAGGG)n FISH demonstrated that repeated units of the two multicopy families were closely associated on the same chromosome pair.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 82 (3), 257-263, 2007
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205473317760
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- NII論文ID
- 10024394522
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 8821849
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可