Identification of gamma ray irradiation-induced mutations in membrane transport genes in a rice population by TILLING
-
- Hwang Jung Eun
- Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI) Division of Ecological Conservation, Bureau of Ecological Research, National Institute of Ecology
-
- Jang Duk-Soo
- Animal and Plant Quarantine Agency
-
- Lee Kyung Jun
- National Agrobiodiversity Center, NAAS, RDA
-
- Ahn Joon-Woo
- Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)
-
- Kim Sang Hoon
- Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)
-
- Kang Si-Yong
- Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)
-
- Kim Dong Sub
- NJ Biopia Co. Ltd.
-
- Kim Jin-Baek
- Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute (KAERI)
この論文をさがす
説明
<p>A high-salt environment represents environmental stress for most plants. Those that can grow and thrive in such an environment must have membrane transport systems that can respond effectively. Plant roots absorb Na+ from the soil, and the plant must maintain Na+ homeostasis to survive salt stress. A major mechanism by which salt-tolerant plants adapt to salt stress is through modulation of ion transport genes. We have subjected a population of rice plants to mutagenesis, and identified lines with both single-nucleotide polymorphisms (SNPs) in membrane transport genes and altered responses to salt stress. Primers labeled with FAM or HEX fluorescent dyes were designed for nine target genes encoding membrane transport proteins that are believed to regulate salt stress tolerance. A TILLING (Targeting Induced Local Lesions IN Genome) assay was performed on 2,961 M2 rice mutant lines using electrophoresis. After the TILLING assay, a total of 41 mutant lines containing SNPs in the target genes were identified and screened. The average number of mutations per gene was 1/492 kb in lines having SNPs, and the percentage of mutation sites per total sequence was 0.67. Among the 41 lines, nine had altered sequences in the exon region of the genes. Of these nine lines, seven were tolerant to salt stress after exposure to 170 mM NaCl for three weeks, while the other two lines were not more salt-tolerant than the control lines. Furthermore, five mutant lines containing SNPs in the coding region of OsAKT1, OsHKT6, OsNSCC2, OsHAK11 and OsSOS1 showed changed expression levels for each gene. We conclude that variation in membrane transport genes, such as expression levels and protein structures, may affect the rice plant’s tolerance to salt stress. These mutations represent traits that may be selected for large rice mutant populations, permitting efficient acquisition of salt-tolerant lines.</p>
収録刊行物
-
- Genes & Genetic Systems
-
Genes & Genetic Systems 91 (5), 245-256, 2016
日本遺伝学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205473444096
-
- NII論文ID
- 130005548832
-
- NII書誌ID
- AA11077421
-
- ISSN
- 18805779
- 13417568
-
- NDL書誌ID
- 028079507
-
- PubMed
- 27582185
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可