Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress
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- Maeda Shunsuke
- Graduate School of Life Sciences, Tohoku University
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- Sakazono Satomi
- Graduate School of Life Sciences, Tohoku University
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- Masuko-Suzuki Hiromi
- Graduate School of Life Sciences, Tohoku University
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- Taguchi Midori
- Graduate School of Life Sciences, Tohoku University
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- Yamamura Kaori
- Graduate School of Life Sciences, Tohoku University
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- Nagano Kuniaki
- Miyagi Prefectural Furukawa Agricultural Experiment Station
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- Endo Takashi
- Miyagi Prefectural Furukawa Agricultural Experiment Station
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- Saeki Kenichi
- Miyagi Prefectural Furukawa Agricultural Experiment Station
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- Osaka Masaaki
- Graduate School of Life Sciences, Tohoku University
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- Nabemoto Moe
- Graduate School of Life Sciences, Tohoku University
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- Ito Kana
- Graduate School of Life Sciences, Tohoku University
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- Kudo Toru
- Faculty of Agriculture, Meiji University
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- Kobayashi Masaaki
- Faculty of Agriculture, Meiji University
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- Kawagishi Mizuho
- Graduate School of Life Sciences, Tohoku University Faculty of Agriculture, Tohoku University
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- Fujita Kotomi
- Graduate School of Life Sciences, Tohoku University Faculty of Agriculture, Tohoku University
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- Nanjo Hikaru
- Graduate School of Life Sciences, Tohoku University Faculty of Agriculture, Tohoku University
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- Shindo Tomoe
- Graduate School of Life Sciences, Tohoku University Faculty of Agriculture, Tohoku University
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- Yano Kentaro
- Faculty of Agriculture, Meiji University
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- Suzuki Go
- Division of Natural Science, Osaka Kyoiku University
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- Suwabe Keita
- Graduate School of Bioresources, Mie University
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- Watanabe Masao
- Graduate School of Life Sciences, Tohoku University
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抄録
<p>Plants subjected to abiotic stress can regulate gene expression post-transcriptionally by means of small RNAs such as microRNAs. Cool-temperature stress causes abnormal tapetum hypertrophy in rice anthers, leading to pollen sterility. As a first step toward understanding the molecular mechanisms of cool tolerance in developing anthers of rice, we report here a comprehensive comparative analysis of microRNAs between cool-sensitive Sasanishiki and cool-tolerant Hitomebore cultivars. High-throughput Illumina sequencing revealed 241 known and 46 novel microRNAs. Interestingly, 15 of these microRNAs accumulated differentially in the two cultivars at the uninucleate microspore stage under cool conditions. Inverse correlations between expression patterns of microRNAs and their target genes were confirmed by quantitative RT-PCR analysis, and cleavage sites of some of the target genes were determined by 5’ RNA ligase-mediated RACE experiments. Thus, our data are useful resources to elucidate microRNA-mediated mechanism(s) of cool tolerance in rice anthers at the booting stage.</p>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 91 (2), 97-109, 2016
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205473609728
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- NII論文ID
- 130005421208
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 027634762
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- PubMed
- 27021915
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可