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- Endo Takashi R.
- Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research Palacký University Olomouc, Centre of the Region Haná for Biotechnological and Agricultural Research
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- Kubaláková Marie
- Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research
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- Vrána Jan
- Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research
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- Doležel Jaroslav
- Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research
この論文をさがす
説明
Despite remarkable recent progress in the analysis of plant genome organization and chromosome structure, there is a need for methods enabling DNA sequences to be mapped by fluorescence in situ hybridization (FISH) at high spatial resolution. We sorted mitotic metaphase chromosomes of wheat by flow cytometry and observed the occurrence of hyperexpanded chromosomes among them. However, this phenomenon was not reproducible in subsequent experiments. An investigation into the procedures of flow cytometry revealed that the hyperexpansion of chromosomes became reproducible when the concentration of formaldehyde used in sample fixation was reduced. We conducted FISH analysis with 45S rDNA, 5S rDNA and wheat centromeric repeat sequences as probes on flow-sorted chromosomes and also on chromosomes from squash preparations. We measured the length of chromosomes 1B and 6B, identified by FISH. On average, the hyperexpanded 1B and 6B chromosomes were 7.26 and 7.53 times longer, respectively, than the same chromosomes from the squash preparations. The most stretched 1B and 6B chromosomes both exceeded 100 micrometers.
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 89 (4), 181-185, 2014
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205473997184
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- NII論文ID
- 130004750049
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 026154994
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- PubMed
- 25747042
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可