Loss- and gain-of-function analyses of vacuolar protein sorting 2 in Notch signaling of Drosophila melanogaster
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- Aoyama Naoki
- Department of Biological Science and Technology, Tokyo University of Science
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- Yamakawa Tomoko
- Department of Biological Science and Technology, Tokyo University of Science Department of Biological Sciences, Graduate School of Science, Osaka University
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- Sasamura Takeshi
- Department of Biological Science and Technology, Tokyo University of Science Department of Biological Sciences, Graduate School of Science, Osaka University
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- Yoshida Yuka
- Department of Biological Science and Technology, Tokyo University of Science
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- Ohori Maki
- Department of Biological Science and Technology, Tokyo University of Science
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- Okubo Hiroyuki
- Department of Biological Science and Technology, Tokyo University of Science
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- Iida Eriko
- Department of Biological Science and Technology, Tokyo University of Science
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- Sasaki Nobuo
- Department of Biological Science and Technology, Tokyo University of Science
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- Ueda Ryu
- Genetic Strains Research Center, National Institute of Genetics
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- Matsuno Kenji
- Department of Biological Science and Technology, Tokyo University of Science Department of Biological Sciences, Graduate School of Science, Osaka University
書誌事項
- タイトル別名
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- Loss- and gain-of-function analyses of <i>vacuolar protein sorting 2</i> in Notch signaling of <i>Drosophila melanogaster</i>
この論文をさがす
抄録
Notch signaling is an evolutionarily conserved mechanism that controls many cell-fate specifications through local cell-cell interactions. The core mechanisms of Notch activation and its subsequent intracellular signaling are well understood. Various cellular functions are required for the activation and regulation of Notch signaling. Among them, the endocytosis of Notch and its ligands is important for the activation and suppression of Notch signaling. The endosomal sorting complex required for transport (ESCRT) proteins are required to sort ubiquitinated membrane proteins, such as Notch, into early endosomes. A loss-of-function allele of vacuolar protein sorting 2 (vps2), which encodes a component of ESCRT-III, has been reported. However, this vps2 mutant still produces the N-terminal half of the protein, and its phenotypes were studied in only a few organs. Here, we generated the first null mutant allele of Drosophila vps2, designated vps22, to better understand the function of this gene. In Drosophila wing imaginal discs homozygous for the vps22 allele, early endosomes and multivesicular bodies (MVBs) were enlarged, and Notch and Delta accumulated inside them. As reported for the previous vps2 mutant, the epithelium grew excessively under this condition. We further studied the roles of vps2 by RNA interference-knockdown. These experiments revealed that a partial reduction of vps2 attenuated Notch signaling; in contrast, the loss-of-function vps2 mutant is reported to up-regulate the Notch signaling in eye imaginal disc cells. These results suggest that Notch signaling can be up- or down-regulated, depending on the level of vps2 expression. Finally, we found that vps2 overexpression also resulted in early-endosome enlargement and the accumulation of Notch and Delta. In these cells, a portion of the Vps2 protein was detected in MVBs and colocalized with Notch. These data indicate that the expression of vps2 must be precisely regulated to maintain the normal structure of early endosomes.
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 88 (1), 45-57, 2013
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205474339840
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- NII論文ID
- 10031183170
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- NII書誌ID
- AA11077421
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- COI
- 1:STN:280:DC%2BC3snktFCksg%3D%3D
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 024439990
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- PubMed
- 23676709
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可