Ty1-copia group retrotransposons in persimmon (Diospyros kaki Thunb.).
-
- Nakatsuka Akira
- Faculty of Life and Environmental Sciences, Shimane University
-
- Iwami Naoko
- Faculty of Life and Environmental Sciences, Shimane University
-
- Matsumoto Shigehito
- Faculty of Life and Environmental Sciences, Shimane University
-
- Itamura Hiroyuki
- Faculty of Life and Environmental Sciences, Shimane University
-
- Yamagishi Masumi
- Faculty of Life and Environmental Sciences, Shimane University
この論文をさがす
抄録
We cloned and characterized Ty1-copia group retrotransposons in persimmon (Diospyros kaki Thunb.). Genomic DNA or methyl jasmonate (MJA)-treated cDNA were used as templates to amplify the reverse transcriptase region of Ty1-copia group retrotransposons. About 280 bp fragments were amplified and cloned, and 97 clones were sequenced. Forty-nine clones included frameshift or the stop codon, or both. Among 48 clones containing complete reading frames, 42 clones had unique nucleotide sequences. Alignment and phylogenetic analysis of putative amino acid sequences in the 42 clones indicated that these clones (named Tdk; retroTransposon in Diospyros kaki) fell into seven subgroups and six ungrouped sequences, indicating high sequence heterogeneity in Tdk clones. Phylogenetic analysis comparing unrelated plant species shows that some Tdk clones are more closely related to Ty1-copia group retrotransposons in the orders Solanales and Sapindales rather than to other Tdk clones. Southern blot analysis using Tdk2B, Tdk4c, Tdk6Ac, Tdk12K and Tdk13G clones as probes showed that persimmon and its related species, D. lotus, D. lotus var. glabba, D. oleifera, D. rhombifolia and D. virginiana, contained multiple Tdk-like sequences, indicating that homologous elements exist in other Diospyros species.<br>
収録刊行物
-
- Genes & Genetic Systems
-
Genes & Genetic Systems 77 (2), 131-136, 2002
日本遺伝学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205474590464
-
- NII論文ID
- 10008639612
-
- NII書誌ID
- AA11077421
-
- COI
- 1:CAS:528:DC%2BD38XmtlGgtb4%3D
-
- ISSN
- 18805779
- 13417568
-
- NDL書誌ID
- 6264876
-
- PubMed
- 12087196
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可