Gic1 is a novel heterochromatin boundary protein in vivo
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- Mitsumori Risa
- Department of Applied Chemistry & Biotechnology, Graduate School of Engineering, University of Fukui
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- Shinmyozu Kaori
- Proteomics Support Unit Center for Developmental Biology, RIKEN
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- Nakayama Jun-ichi
- Graduate School of Natural Sciences, Nagoya City University
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- Uchida Hiroyuki
- Department of Applied Chemistry & Biotechnology, Graduate School of Engineering, University of Fukui
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- Oki Masaya
- Department of Applied Chemistry & Biotechnology, Graduate School of Engineering, University of Fukui Research and Education Program for Life Science, University of Fukui PRESTO, Japan Science and Technology Agency (JST)
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説明
<p>In Saccharomyces cerevisiae, HMR/HML, telomeres and ribosomal DNA are heterochromatin-like regions in which gene transcription is prevented by the silent information regulator (Sir) complex. The Sir complex (Sir2, Sir3 and Sir4) can spread through chromatin from the silencer. Boundaries prevent Sir complex spreading, and we previously identified 55 boundary genes among all ~6,000 yeast genes. These boundary proteins can be distinguished into two types: those that activate transcription to prevent spreading of silencing, and those that prevent gene silencing by forming a boundary. We selected 44 transcription-independent boundary proteins from the 55 boundary genes by performing a one-hybrid assay and focused on GIC1 (GTPase interaction component 1). Gic1 is an effector of Cdc42, which belongs to the Rho family of small GTPases, and has not been reported to function in heterochromatin boundaries in vivo. We detected a novel boundary-forming activity of Gic1 at HMR-left and telomeric regions by conducting a chromatin immunoprecipitation assay with an anti-Sir3 antibody. We also found that Gic1 bound weakly to histones in two-hybrid analysis. Moreover, we performed domain analysis to identify domain(s) of Gic1 that are important for its boundary activity, and identified two minimum domains, which are located outside its Cdc42-binding domain.</p>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 91 (3), 151-159, 2016
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205474803456
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- NII論文ID
- 130005170330
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 027722950
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- PubMed
- 27301280
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可