各種抗癌剤の膀胱癌の核酸代謝に対する影響について

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タイトル別名
  • THE EFFECTS OF VARIOUS ANTI-CANCER DRUGS ON NUCLEIC ACID METABOLISM OF HUMAN BLADDER CANCER <i>IN VITRO</i>
  • カクシュ コウガンザイ ノ ボウコウガン ノ カクサン タイシャ ニ タイスル

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The effects of various anti-cancer drugs on nucleic acid metabolism of human bladder tumor were investigated in vitro. After incubating the slices of human bladder tumor with 5-fluorouracil (5-FU), mitomycin C (MMC) or cyclophosphamide, combination of either two drugs or three drugs, the slices were homogenized and fractionized. Nuclear, mitochondrial and microsomal fractions were obtained. 5-FU, MMC or cyclophosphamide inhibited the incorporation rate of adenine-8-C14 into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) of the nuclear fraction of human bladder cancer. 5-FU, MMC and cyclophosphamide had no additi ve inhibitory effects on the incorporation rate of adenine-8-C14 into DNA and RNA of the nuclear fraction, when either two drugs were given together or three drugs were given to the incubation medium. The incorporation rate of adenine-8-C14 into DNA of the mitochondrial fraction of human bladder cancer was inhibited by 5-FU, cyclophosphamide or MMC. When either two drugs or combination of these three drugs were given to the incubation medium, these drugs had no additive inhibitory effects on the incorporation rate of adenine-8-C14 into DNA of the mitochondrial fraction of human bladder cancer. Sinilar result was obtained with the microsomal fraction. These in vitro experiments would provide us with a screening system of anticancer drugs and help to bridge a gap between the results of animal experiments and those of clinical application of the anti-cancer drugs.

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