Cellular and genetic analysis of a chimera in dizygote twins

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  • Atoh M
    Department of Laboratory Medicine, and 2)Department of Blood Transfusion, National Defense Medical College
  • Kobayashi K
    Department of Laboratory Medicine, and 2)Department of Blood Transfusion, National Defense Medical College
  • Suzuki H
    防衛医科大学校輸血部
  • Suzuki Y
    防衛医科大学校輸血部
  • Kaneko T
    防衛医科大学校輸血部
  • Xxxxx Xxxxx
    防衛医科大学校法医学講座
  • Ishigami S
    越谷市立病院臨床検査科
  • Fukuda Y
    越谷市立病院臨床検査科
  • Tamai S
    Department of Laboratory Medicine, and 2)Department of Blood Transfusion, National Defense Medical College

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Other Title
  • 二卵性双生児にみられたキメラの細胞学的ならびに遺伝子学的解析

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<p>We found a case that chimerism was suspected in ABO blood typing and performed flow cytometry analysis, microsatellite analysis, DNA typing for the HLA-A, B, C, DRB1 loci and the ABO blood group gene to establish a chimerism. This case is a 37-year-old male, and he has a dizygotic twins' younger brother. He was hospitalized with the fracture of his distal left radius. There is no history of blood transfusion. We tested the ABO blood group using a flow cytmetry. We extracted genomic DNA from peripheral blood, and then analysed microsatellites, the HLA-A, B, C, DRB, DQB1 loci and the ABO blood group locus. The flow cytmetry tests showed that the red cells with the B and the AB blood type was 90.04% and 9.96%, respectively. Three or four kinds of alleles were detected by the DNA typings and microsatellite analyses. From these findings, it was suggested that this case is a chimera in dizygote twins.</p>

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