Histochemical Study on the Bile Duct System of Normal Mongolian Gerbils

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  • ITAGAKI Shin-ichi
    <I>Department of Biomedical Science, Faculty of Agriculture, The University of Tokyo</I>
  • PETRUCCELLI Miguel A.
    <I>Department of Biomedical Science, Faculty of Agriculture, The University of Tokyo</I>
  • DOI Kunio
    <I>Department of Biomedical Science, Faculty of Agriculture, The University of Tokyo</I>

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Other Title
  • 正常スナネズミ胆管系の組織化学的研究
  • 正常スナネズミ胆管系の組織化学的研究〔英文〕
  • セイジョウ スナネズミ タンカンケイ ノ ソシキ カガクテキ ケンキュウ エイ

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Abstract

The bile duct system of normal Mongolian gerbils was examined histochemically. The luminal surface membrane and apical cytoplasm of the biliary and gallbladder epithelial cells were stained with periodic acid-Schiff (PAS), alcian blue, pH 2.5 (AB) and high iron diamine (HID) -AB, and many epithelial cells of the common bile duct and gallbladder had weakly PAS-positive granular material in their supranuclear cytoplasm. Lectin-histochemiaclly, these cells had binding sites to Concanavalia ensiformis (ConA), Dolichos biflorus (DBA), Glycine max (SBA), Ulex europeas-I (UEA-I), and Triticum vulgaris (WGA), On the other hand, the periductal glandular epithelial cells were not stained by any histochemical stainings. In addition to these light microscopic findings, the electron microscopic findings based on the periodic acid-silver methenamine method and avidinbiotin colloidal gold method for DBA and WGA suggested that the biliary and gallbladder epithelial cells of Mongolian gerbils secreted mucin with terminal sialic and sulfonic acid residues and that the lectin binding activity of mucin secreted from these cells was similar to that of mucin secreted from the periductal glandular epithelial cells of mice and rats.

Journal

  • Experimental Animals

    Experimental Animals 40 (2), 153-160, 1991

    Japanese Association for Laboratory Animal Science

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