Ovicadal Fffects of Heat and Disinfectants on <I>Syphacia muris</I> Estimated by <I>In Vitro Hatching</I>

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  • MIYAJI Suguru
    <I>First Department of Surgery, Teikyo University School of Medicine</I>
  • KAMIYA Masao
    <I>Department of Parasitology, Faculty of Veterinary Medicine, Hokkaido University</I>
  • SHIKATA Jun-ichi
    <I>First Department of Surgery, Teikyo University School of Medicine</I>

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Other Title
  • 人工孵化法による<I>Syphacia muris</I>虫卵に対する熱および各種消毒薬の殺卵効果
  • 人工孵化法によるSyphacia muris虫卵に対する熱および各種消毒薬の殺卵効果
  • ジンコウ フカホウ ニ ヨル Syphacia muris チュウラン ニ タ

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Abstract

The ovicidal effects of heat and various chemical disinfectants on an oxyurid rat nematode Syphacia muris were investigated, using the hatching methods in artificial intestinal juice. The eggs were collected from the perianal skin of spontaneously infected rats by means of a piece of transparent adhesive tapes, and these eggs were treated with each disinfectant for two hours. It was found that 70% ethanol and 80°C 30 min treatments killed almost all of the eggs. However, a small number of the eggs tested was killed by 0.02% chlorhexidine digluconate or 0.05% benzethonium chloride. AlcideTM, 3% saponated cresol solution, 50% isopropanol, 10 ppm sodium hypochlorite and 5 ppm iodophol had some effects against the eggs, but they didn't kill the eggs completely. A biological assay through infection of the eggs to rats might be necessary because the effects of 2% formalin on the eggs were not determined by the hatching methods.

Journal

  • Experimental Animals

    Experimental Animals 37 (4), 399-404, 1988

    Japanese Association for Laboratory Animal Science

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