Improvement of Cardiac Puncture in Mice

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  • OHWADA Kazuo
    <I>Laboratory Animal Center, Yamagata University School of Medicine</I>

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Other Title
  • マウス心臓採血法の改良
  • マウス シンゾウ サイケツホウ ノ カイリョウ

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Description

Cardiac puncture through the anterior thoracic aperture, which was originally reported by Frankeberg, was improved. The improved method involved the use of a restraining board instead of manual holding. A mouse anesthetized with ether was fixed in a dorsal position on the restraining board, and then a needle of 26G×1/2 was inserted through the skin just in front of the anterior thoracic aperture, keeping the syringe parallel to the long axis of the animal. A blood volume of 0.6-0.9 ml could be obtained even by an inexperienced collector, and the blood collection was usually completed within 10-20 s. The potential for repeated blood collection of 0.4-0.6 ml was facilitated by use of the present method at intervals of 7 days or more.

Journal

  • Experimental Animals

    Experimental Animals 35 (3), 353-355, 1986

    Japanese Association for Laboratory Animal Science

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