Methods for Intravenous Injection and Blood Collection in the Guinea Pig via Veins of the Forelimb without Anesthesia

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  • TAKAHASHI Kazuaki W.
    <I>Laboratory of Laboratory Animal Science, Nippon Veterinary and Zootechnical College</I>
  • SAKUMA Shinichi
    <I>Department of Physiology, Nippon Veterinary and Zootechnical College</I>
  • UEDA Hideo
    <I>Department of Physiology, Nippon Veterinary and Zootechnical College</I>
  • EBINO Koichi Y.
    <I>Toxicology Division, The Institute of Environmental Toxicology</I>
  • SAITO Toru R.
    <I>Toxicology Division, The Institute of Environmental Toxicology</I>
  • IMAMICHI Tomonori
    <I>Department of Physiology, Nippon Veterinary and Zootechnical College</I>

Bibliographic Information

Other Title
  • モルモットの前肢の静脈を用いた無麻酔による静脈内注射と採血法
  • モルモット ノ ゼンシ ノ ジョウミャク オ モチイタ ム マスイ ニヨル ジ

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Description

Methods for intravenous injection and blood collection by way of the forelimb in guinea pigs without anesthesia were investigated, with the results as follows: 1) Both the accessory cephalic and cephalic veins were found available for injection as well as for drawing blood samples. Venipuncture was accomplished with greater ease by using the accessory cephalic vein. 2) The following procedure has proven practical. (i) Clip the hair over the site. (ii) Restrain the animal to immobilize the foreleg in slight extension by grasping it at the elbow joint with the thumb and forefinger over its dorsal and ventral aspects, respectively. (iii) After disinfecting the skin surface with alcohol, perform injection using an appropriate syringe with a 27 (1/4) or 25 (1/3) gauge intravenous needle, or puncture the vein with a 23 (1/2) gauge i.v. needle to collect the blood that drips out.

Journal

  • Experimental Animals

    Experimental Animals 29 (3), 351-354, 1980

    Japanese Association for Laboratory Animal Science

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