Effect of Proximate Environment on Drug Susceptibility of Mice

TANASE Hisao, MATSUNUMA Naochika, SUZUKI Yoshio

doi:10.1538/expanim1978.28.4_507

The present study was performed in order to elucidate the effect of proximate environment on drug susceptibility of mice. Three experiments were carried out independently. In the first experiment, mongrel and ddS mice produced under an unsatisfactory control of proximate environment were purchased, and acute toxicity tests of thiamine hydrochloride (B₁HCl) and isonicotinic acid hydrazide (INAH) were practiced at two different conditioned rooms. In the second experiment, ddY mice produced under the conventional environment controlled to a certain extent were purchased, and the toxic effect of B₁HCl was examined under the similar environment. In the third experiment, the sensitivity to B₁HCl of RFVL mice produced under the strict barrier system was tested at the severe air-conditioned room. LD₅₀ and their fLD₅₀ values were calculated by Litchfield-Wilcoxon's method, and the variance analyses were carried out. The severer the environmental control after the purchase of mice turned to the higher the drug sensitivity. This respect was more remarkable in INAH of which the toxic response is appeared slowly compared with B₁HCl. Furthermore, seasonal variation was found in LD₅₀ values. However, seasonal effect differed from rearing and experimental conditions. In the third experiment which these proximate environments were controlled severely, seasonal variation was very small. From the results of these experiments, it was defined that the use of animals produced under the satisfactory rearing condition and severe environmental control are necessary for animal experiments.

Effect of Proximate Environment on Drug Susceptibility of Mice

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