Three-dimensional characterization of osteoclast bone-resorbing activity in the resorption lacunae

DOI 機関リポジトリ PubMed
  • Niroshani Surangika Soysa
    Section of Pharmacology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Japan
  • Neil Alles
    Section of Pharmacology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Japan Global COE program, Tokyo Medical and Dental University, Japan.
  • 青木 和広
    Section of Pharmacology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Japan
  • 大谷 啓一
    Section of Pharmacology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Japan

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説明

Confocal laser microscopy is a well-recognized research tool in the fields of biological and material science which enables high-resolution images of samples with minimum requirements for specimen preparation. Here we introduce an innovative technique for the 3-D description and measurement of resorption pits using Super Depth Surface Profile Measurement Microscope based on the principle of confocal microscope. We show one example of culturing for 48 h with an established NF-κB inhibitor named NBD-peptide after plating mature osteoclasts on dentine slices with osteoblasts. The activity of osteoclasts is measured by determining the volume of resorbed portion of dentine by osteoclasts in vitro. The 3-D surface profile could be obtained by detecting the position at which the reflected laser intensity from the target becomes the maximum on z-axis. The volume and depth of resorption lacunae by stimulated osteoclasts is significantly increased compared to the un-stimulated group without changing of resorption area. The increase in volume and depth are dose-dependently inhibited by the NBD-peptide. Comparing to the classical method by measuring 2-D area of pits, analysis based on this technique could provide reliable quantitative assessment reflecting the osteoclast activity.

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詳細情報 詳細情報について

  • CRID
    1390001205517154048
  • NII論文ID
    130005420250
    120006409934
  • NII書誌ID
    AA12028964
  • DOI
    10.11480/jmds.560302
  • ISSN
    21859132
    13428810
  • PubMed
    20099473
  • Web Site
    http://id.nii.ac.jp/1569/00000098/
  • 本文言語コード
    en
  • 資料種別
    departmental bulletin paper
  • データソース種別
    • JaLC
    • IRDB
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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