Retraction: Inflammatory Cytokine-induced MMP-3 Regulates Proliferation of Odontoblast-like Cells Derived from Induced Pluripotent Stem Cells
-
- Nobuaki OZEKI
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Hideyuki YAMAGUCHI
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Taiki HIYAMA
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Naoko HASE
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Rie KAWAI
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
-
- Makio MOGI
- Department of Medicinal Biochemistry, School of Pharmacy, Aichi Gakuin University
-
- Kazuhiko NAKATA
- Department of Endodontics, School of Dentistry, Aichi Gakuin University
Bibliographic Information
- Other Title
-
- 論文撤回:炎症性サイトカイン誘導MMP-3はマウスiPS細胞由来象牙芽細胞の増殖を制御する
Search this article
Description
Purpose: We previously reported that matrix metalloproteinase (MMP) -3 accelerates wound healing following dental pulp injury in rats. Additionally, we identified that a proinflammatory cytokine mixture (CM; interleukin-1β, tumor necrosis factor-α, and interferon-γ) induced MMP-3 activity in odontoblast-like cells derived from mouse embryonic stem cells, suggesting that MMP-3 plays a potentially unique role in wound healing and dental pulp regeneration in odontoblast-like cells. Here, we investigate whether upregulation of MMP-3 activity by CM regulates the proliferation and apoptosis of purified odontoblast-like cells derived from induced pluripotent stem (iPS) cells.<br> Methods: The proportion of α2 integrin-positive cells in the total differentiated odontoblast-like cell population is a measure of the purity of the iPS cell-derived odontoblast-like cells, and was estimated by FACS analysis to be 98%. Reverse transcriptase polymerase chain reaction, western blotting, an MMP-3 activity assay, a BrdU cell proliferation enzyme-linked immunosorbent assay, and DNA fragmentation analysis were used to evaluate siRNA-mediated down-regulation of MMP-3 expression and activity, and any changes in the proliferative and apoptotic responses associated with this reduced expression.<br> Results: Low concentrations of CM (1 or 3U) induced MMP-3 mRNA and protein expression, increasing MMP-3 activity and cell proliferation (p<.05), but not apoptosis. MMP-3 silencing produced a potent and significant suppression of cytokine-induced MMP-3 expression and activity, decreased cell proliferation and increased apoptosis (p<.05). These effects were restored by application of exogenous MMP-3 (p<.05).<br> Conclusion: Current evidence suggests that low concentrations of CM induce an MMP-3-mediated increase in odontoblast-like cell proliferation, whereas higher concentrations (5U) inhibit cell proliferation and promote apoptosis. Our current data suggests that CM induces MMP-3-regulated cell proliferation and anti-apoptosis effects in odontoblast-like cells derived from iPS cells, in addition to well-documented destructive roles in inflammation.
Journal
-
- The Japanese Journal of Conservative Dentistry
-
The Japanese Journal of Conservative Dentistry 57 (4), 358-368, 2014
The Japanese Society of Conservative Dentistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001205520436224
-
- NII Article ID
- 130004687192
-
- ISSN
- 21880808
- 03872343
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- CiNii Articles
-
- Abstract License Flag
- Disallowed