Inhibitory Effects of Cryptotanshinone on Inflammation-related Molecules in Human Gingival Fibroblasts

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  • Koji NARUISHI
    Department of Periodontology, Tokushima University Hospital
  • Yukari KAJIURA
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • Yasufumi NISHIKAWA
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • Mika BANDO
    Department of Periodontology, Tokushima University Hospital
  • Jun-ichi KIDO
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • Toshihiko NAGATA
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School

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  • 培養歯肉線維芽細胞におけるクリプトタンシノンによる炎症関連分子の産生抑制効果

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 Purpose: Cryptotanshinone (CPT) isolated from the root of an Asian medicinal plant, Salvia miltiorrhiza bunge, acts as a potent anti-tumor or anti-inflammatory agent in vitro. However, the effects of CPT on the progression of periodontitis are still unknown. This study investigated the effects of CPT on the production of inflammation-related molecules in human gingival fibroblasts.<br> Methods: Human gingival fibroblasts (HGFs), CRL-2014TM (ATCC), were used. Cytotoxicity of the cells by CPT was examined. Dead cells stained by trypan blue were counted and analyzed. Next, the levels of IL-6 and VEGF in IL-1β-treated cells with or without CPT were measured using ELISA kits. The productivity of cathepsin L in IL-1β-treated cells with or without CPT was examined using Western blotting.<br> Results: The ratio of viable cell numbers was significantly decreased by 100 μmol/l CPT. Next, the increase of IL-6 and cathepsin L induced by IL-1β was significantly suppressed by the pretreatment of 10 μmol/l CPT in HGFs. On the other hand, productivity of VEGF induced by IL-1β was not changed by the pretreatment of 10 μmol/l CPT.<br> Conclusion: CPT suppresses IL-1β-induced IL-6 and cathepsin L production in HGFs, and might regulate the progression of periodontitis.

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