Osmotic Stress Affects Calcium Deposition and Osteopontin Expression in Cultured Rat Dental Pulp Cells

DOI 28 References
  • INAGAKI Yuji
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • BANDO Mika
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • NAKAJIMA Yukiko
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • HIROSHIMA Yuka
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • KIDO Jun-ichi
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School
  • NAGATA Toshihiko
    Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School

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  • オスモティックストレスが培養歯髄細胞の硬組織形成能とオステオポンチン産生に及ぼす影響

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Abstract

Osmotic stress is a stimulus caused by osmotic pressure, which is known to affect physiological functions in various tissues and cells. However, there is little information on the effect of osmotic stress on dental pulp tissues and cells. In this study, we investigated the relationship between osmotic stress and calcium deposition in cultured rat dental pulp cells. We also analyzed the effect of osmotic stress on the expression and phosphorylation of osteopontin (OPN), a multi-functional bone matrix protein, in the dental pulp cells. Both hypo- and hyper-osmotic stress affected cell-shape and -growth, and decreased calcium deposition in cultured rat dental pulp cells. Hypoosmotic stress increased OPN secretion and its protein ratio in the culture medium. Also, the secreted OPN was highly phosphorylated. On the other hand, hyper-osmotic stress decreased OPN secretion and its protein ratio and most of the OPN in the hypertoinc medium was dephosphorylated. Both hypo- and hyper-osmotic stress decreased calcium deposition in the cultured rat dental pulp cells. However, the mechanism of the inhibitory action seemed to be different in the two groups, because the amount and phosphorylation pattern of OPN were not similar between the hypo- and hyper-osmotic conditions. We concluded that osmotic stress affects calcium deposition in cultured rat dental pulp cells and that OPN expression and its phosphorylation may be a control factor of osmotic stress-associated mineralization.

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