Peptidomic Analysis in Sera from Patients with Systemic Sclerosis (SSc): Complement DRC3f is dominantly detected in SSc and enhances proliferation of vascular endothelial cells
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- 向 陽
- Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
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- 松井 利浩
- Department of Rheumatology, Sagamihara National Hospital, National Hospital Organization
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- 松尾 光祐
- Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
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- 島田 浩太
- Department of Rheumatology, Sagamihara National Hospital, National Hospital Organization
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- 當間 重人
- Department of Rheumatology, Sagamihara National Hospital, National Hospital Organization
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- 中村 洋
- Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
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- 増子 佳世
- Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
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- 遊道 和雄
- Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
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- 西岡 久寿樹
- Department of Frontier Medicine, Institute of Medical Science, St. Marianna University School of Medicine
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- 加藤 智啓
- Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
抄録
Objective: To find the biomarker of SSc at peptide level and to investigate the biological function of the molecule.<BR>Methods: Sera form 40 patients with SSc, 30 SLE, 21 RA, 30 OA, and 26 healthy donors were used in peptidomic analysis. The biofunctions of DRC3f and C3f was testified by stimulating the cultured skin fibroblasts, skin and lung microvascular endothelial cells (MEC). The cell proliferation was observed on bioreduction of MTS into formazan by living cells. The production of TGF-beta, VEGF and EGF were measured by ELISA.<BR>Results: A group of peptides was detected dominantly in SSc sera and identified to be DRC3f and its derivatives. The levels of DRC3f was related to the severity and activity of SSc. Synthesized DRC3f and C3f enhanced the MEC proliferation independent on growth factors. Both the whole and filtered sera containing DRC3f enhanced proliferation of endothelial cells. Increased production of TGF-beta by DRC3f was also observed in skin fibroblasts and MEC.<BR>Conclusions: DRC3f may be a useful marker of SSc and may play important roles in pathogenesis of SSc.
収録刊行物
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- 日本臨床免疫学会総会抄録集
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日本臨床免疫学会総会抄録集 34 (0), 34-34, 2006
日本臨床免疫学会
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詳細情報 詳細情報について
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- CRID
- 1390001205524886656
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- NII論文ID
- 130006949094
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- ISSN
- 18803296
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可