Peptidomic Analysis in Sera from Patients with Systemic Sclerosis (SSc): Complement DRC3f is dominantly detected in SSc and enhances proliferation of vascular endothelial cells

DOI
  • Xiang Yang
    Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
  • Matsui Toshihiro
    Department of Rheumatology, Sagamihara National Hospital, National Hospital Organization
  • Matsuo Kosuke
    Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
  • Shimada Kota
    Department of Rheumatology, Sagamihara National Hospital, National Hospital Organization
  • Tohma Shigeto
    Department of Rheumatology, Sagamihara National Hospital, National Hospital Organization
  • Nakamura Hiroshi
    Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
  • Masuko Kayo
    Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
  • Yudoh Kazuo
    Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine
  • Nishioka Kusuki
    Department of Frontier Medicine, Institute of Medical Science, St. Marianna University School of Medicine
  • Kato Tomohiro
    Department of Bioregulation and Proteomics, Institute of Medical Science, St. Marianna University School of Medicine

Description

Objective: To find the biomarker of SSc at peptide level and to investigate the biological function of the molecule.<BR>Methods: Sera form 40 patients with SSc, 30 SLE, 21 RA, 30 OA, and 26 healthy donors were used in peptidomic analysis. The biofunctions of DRC3f and C3f was testified by stimulating the cultured skin fibroblasts, skin and lung microvascular endothelial cells (MEC). The cell proliferation was observed on bioreduction of MTS into formazan by living cells. The production of TGF-beta, VEGF and EGF were measured by ELISA.<BR>Results: A group of peptides was detected dominantly in SSc sera and identified to be DRC3f and its derivatives. The levels of DRC3f was related to the severity and activity of SSc. Synthesized DRC3f and C3f enhanced the MEC proliferation independent on growth factors. Both the whole and filtered sera containing DRC3f enhanced proliferation of endothelial cells. Increased production of TGF-beta by DRC3f was also observed in skin fibroblasts and MEC.<BR>Conclusions: DRC3f may be a useful marker of SSc and may play important roles in pathogenesis of SSc.

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Details 詳細情報について

  • CRID
    1390001205524886656
  • NII Article ID
    130006949094
  • DOI
    10.14906/jscisho.34.0.34.0
  • ISSN
    18803296
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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