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- YAMAMOTO Yasuhisa
- Department of Surgery. Okayama University Medical School
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- NAKASHIMA Yoichi
- Department of Surgery. Okayama University Medical School
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- OKANO Yutaka
- Department of Surgery. Okayama University Medical School
Bibliographic Information
- Other Title
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- Antibody formation for malignant tumor-1-Antitumor activity of the regional lymph node protein
- I. ANTITUMOR ACTIVITY OF THE REGIONAL LYMPH NODE PROTEIN
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Abstract
Lymphoid cells in regional lymph nodes of mice bearing Ehrlich ascites cell tumor have some cytocidal action on cultured Ehrlich ascites carcinoma cells. The regional lymph node protein was fractionated on Sephadex G-200 column and on DEAE-Sephadex A-50 column. The antitumor activity was determined by cell culture test. The antitumor activity was mainly present in the fraction with β-mobility from DEAE-Sephadex A-50 column chromatography. The sedimentation coefficient of this protein was 6.5S. This protein was soluble and permeable through a cell-impermeable diffusion chamber. The minimum protein concentration required for suppression of tumor cell growth was about 300μg for 104 cultured JTC-11 cells in 10ml culture vessel. In electrophoresis, this fraction moved mainly to β-position, and contained a small amount of α-band. The antibody in regional lymph node was loaded mainly on β- and α-mobility protein.
Journal
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- GANN Japanese Journal of Cancer Research
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GANN Japanese Journal of Cancer Research 59 (2), 109-116, 1968
The Japanese Cancer Association
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Details 詳細情報について
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- CRID
- 1390001205529042688
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- NII Article ID
- 130006408692
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- NII Book ID
- AN00045888
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- NDL BIB ID
- 8148433
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- ISSN
- 0016450X
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed