SURFACE ANTIGENIC CHARACTERISTICS OF HUMAN MELANOMA CELLS DEFINED BY XENOANTISERUM RAISED AGAINST PAPAIN-SOLUBILIZED MELANOMA-ASSOCIATED ANTIGENS
-
- 石井 良文
- Department of Developmental Therapeutics, M. D. Anderson Hospital and Tumor Institute, The University of Texas System Cancer Center Department of Pathology, Sapporo Medical College
-
- GOLDHOFER Werner
- Department of Developmental Therapeutics, M. D. Anderson Hospital and Tumor Institute, The University of Texas System Cancer Center
-
- MAVLIGIT Giora M.
- Department of Developmental Therapeutics, M. D. Anderson Hospital and Tumor Institute, The University of Texas System Cancer Center
書誌事項
- タイトル別名
-
- Surface Antigenic Characteristics of Hu
この論文をさがす
抄録
Xenoantiserum to human malignant melanoma was prepared by immunizing rabbits with melanoma associated antigens (MAA) solubilized from melanoma cell membrane by limited papain digestion. The antiserum was absorbed extensively with red cells, leukemia cells and cultured lymphoid cell lines, and was assayed for its reactivity with different human cell types by the indirect membrane immunofluorescence and radioimmunoprecipitation techniques. The data obtained suggest that there are at least two different MAA on human melanoma cells. The first is melanoma-group specific and can be detected commonly on different melanoma cell lines. The second is oncofetal and is shared by melanoma, carcinoma and fetal cells tested thus far. Immunoprecipitated material from melanoma cell membrane that had been radioiodinated with lactoperoxidase and solubilized with a non-ionic detergent was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which showed two major peaks with estimated molecular weights of 90, 000 and 120, 000 daltons. The 90, 000 molecular weight component appears to be oncofetal, as it disappeared when the antiserum was absorbed with either melanoma or carcinoma cell lines.
収録刊行物
-
- GANN Japanese Journal of Cancer Research
-
GANN Japanese Journal of Cancer Research 71 (6), 881-888, 1980
日本癌学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205530739200
-
- NII論文ID
- 130006410128
- 40000529551
-
- NII書誌ID
- AN00045888
-
- NDL書誌ID
- 2339229
-
- ISSN
- 0016450X
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可