ANALYSIS OF THE HYDROGEN FERMENTATIVE MICROBIAL COMMUNITY BY USING PCR-DGGE METHOD
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- TOSAKA Michihiro
- 東北大学 大学院工学研究科
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- LI Yu-You
- 東北大学 大学院工学研究科
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- NOIKE Tatsuya
- 東北大学 大学院工学研究科
Bibliographic Information
- Other Title
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- PCR-DGGE法を用いた水素発酵微生物群集の構造解析
- PCR DGGEホウ オ モチイタ スイソ ハッコウ ビセイブツ グンシュウ ノ コウゾウ カイセキ
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Description
A Continuous experiment on the hydrogen fermentation of glucose was conducted to investigate the relationship between the hydrogen productivity and the microbial community of the mixed micro-flora. A total of five different kinds of inoculums including digested sludge, heat treated digested sludge, activated sludge, compost and a special consortium were used as the seeds for five chemostat-type reactors. Each reactor was continuously operated at the same condition of 35°C, a hydraulic retention time 12 hrs and a pH5.5 using a medium with glucose of 10000 mg/L for over two months. It was found that the fermentative pattern and the hydrogen productivity depend on the initial inoculums. The analysis results of PCR-DGGE method and DNA sequence indicates that Clostridium acetobutylicum and Clostridium pasteurianum were the important bacteria in hydrogen fermentation for obtaining a high H2 yield.
Journal
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- Doboku Gakkai Ronbunshu
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Doboku Gakkai Ronbunshu 2005 (790), 790_1-790_10, 2005
Japan Society of Civil Engineers
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Keywords
Details 詳細情報について
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- CRID
- 1390001205601256704
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- NII Article ID
- 10014336713
- 10021088655
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- NII Book ID
- AN10014020
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- ISSN
- 18827187
- 02897806
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- NDL BIB ID
- 7314716
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed