MICROBIAL COMMUNITY ANALYSIS IN THREE ANAEROBIC/AEROBIC ACTIVATED SLUDGE PROCESSES FED WITH ACETATE, DOMESTIC SEWAGE WITH AND WITHOUT PARTICULATE ORGANIC MATTER

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  • LEE Mikyung
    社団法人 土木学会 東京大学 大学院工学系研究科都市工学専攻
  • NAKAJIMA Fumiyuki
    社団法人 土木学会 東京大学 大学院工学系研究科
  • FURUMAI Hiroaki
    社団法人 土木学会 東京大学 大学院工学系研究科

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  • 酢酸, 都市下水および懸濁性有機物を添加した都市下水を処理する嫌気好気活性汚泥の微生物群集構造解析
  • サクサン トシ ゲスイ オヨビ ケンダクセイ ユウキブツ オ テンカ シタ トシ ゲスイ オ ショリ スル ケンキ コウキカッセイ オデイ ノ ビセイブツ グンシュウ コウゾウ カイセキ

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Abstract

Two SBR reactors were operated with feed of domestic sewage (Run-S) and enriched sewage with particulate organic matters (Run-SP) until stable EBPR process was developed, while the third reactor was fed with acetate (Run-A) for a reference. Microbial community of activated sludges was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method. Based on the analysis of DGGE band patterns, three bands were found commonly in all the lanes. Four bands were common between Run-A and Run-SP. There was no remarkable difference in band patterns between Run-A and Run-SP, which indicated that the addition of POM did not affect significantly the microbial community change in the activated sludge. The DNA sequences of the bands C1 and A/SP corresponded to the related sequences of Thiothrix sp. and Rhodocyclus sp., respectively.

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