Function of apoplastic glycosyl hydrolases induced by sugar starvation in Arabidpsis
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- Lee Eun-Jeong
- Reserach and Education Center for Genetic Information, Nara Institute of Science and Technology
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- Sano Hiroshi
- Reserach and Education Center for Genetic Information, Nara Institute of Science and Technology
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- Koizumi Nozomu
- Reserach and Education Center for Genetic Information, Nara Institute of Science and Technology
Bibliographic Information
- Other Title
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- 糖欠乏により誘導されるシロイヌナズナの細胞壁型グリコシダーゼの生理機能
Abstract
In order to understand carbohydrate metabolism regulated by sugar, we screened genes whose expression was regulated by sucrose using cDNA macro-arrays of Arabidopsis. In this study, we focused genes encoding putative beta-galactosidase, beta-xylosidase and beta-glucosidase induced by sugar starvation and designated them as GAL, XYL and GLU, respectively. They have glycosyl hydrolase domains and signal peptides. GAL showed high homology with a beta-galactosidase induced during fruit ripening of pear. Thus, we hypothesized that those proteins secrete and release monosaccharides from cell wall polysaccharides. In fact, the activity of glycosyl hydrolases in culture medium increased when sucrose was omitted from culture medium. Use of GUS reporter gene indicated activation of GAL promoter by dark treatment. In addition, cell wall polysaccharides such as hemicellulose and pectin decreased in sugar-starved leaves. Thus, it was indicated that cell wall polysaccharides are degraded to supply carbon source associating with sugar starvation.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2005 (0), 161-161, 2005
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205626592256
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- NII Article ID
- 130006986676
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed