Zn-Chl a is Present in PSI and PSII Isolated from <I>Chlorella</I> Cells Grown in the Dark with the Excessive Amounts of Zn<SUP>2+</SUP>

DOI

Bibliographic Information

Other Title
  • 高濃度亜鉛存在下で生育したクロレラ細胞が形成するZn-Chl aはPSIおよびPSIIに結合している

Abstract

Zn-Chl a was formed in Chlorella grown in the dark with the excessive Zn2+. (1) The membrane fragments isolated from Zn-grown Chlorella were solubilized with Triton X-100 or β-dodecylmaltoside, and fractionated by centrifugation into the precipitates at 30,000rpm for 20 min (30kppt) and at 50,000rpm for 60 min (50kppt), and its supernatant (50ksup). (2) P700/Chl a ratios suggest that PSII and PSI were enriched in 30kppt and in 50kppt, respectively, while LHCP was enriched in 50ksup. (3) The low-temperature fluorescence indicated that the far-red fluorescence was not dominant in 50kppt, probably due to the labile far-red Chl forms in 50kppt. (4) Zn-Chl a/Chl a ratios were almost the same in 30kppt and in 50kppt, but extremely low in 50ksup, suggesting that Zn-Chl a was equally incorporated into PSII and PSI. (5) PSI and PSII bands were not so clear on the SDS-PAGE, suggesting their easy fragmentation during SDS-PAGE.

Journal

Details 詳細情報について

  • CRID
    1390001205627101696
  • NII Article ID
    130006987249
  • DOI
    10.14841/jspp.2005.0.619.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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