The Difference of Heavy Metal Accumulation and Protein Pattern between <I>Chlamydomonas acidophila</I> DVB 238 and <I>Chlamydomonas acidophila</I> KT-1

DOI

Bibliographic Information

Other Title
  • 日本産およびイタリア産の好酸性緑藻における重金属蓄積機構関連タンパク質の比較と探索

Abstract

Acidophilic algae, Chlamydomonas acidophila DVB 238 and Chlamydomonas acidophila KT-1, had very strong heavy metal tolerance in comparison with other neutrophilic algae. To explore the possibility for use of phytoremediation, two acidophilic algae were investigated the accumulation of metal (Cd and Ni) and the protein synthesis under various metal concentration.<br>C. acidophila DVB 238 showed higher accumulation (13.7 μg g Cd and 2.59 μg g Ni per 108 cells) than C. acidophila KT-1. Moreover Cd treatment did not induce an increase of protein in C. acidophila KT-1 but C. acidophila DVB 238. Therefore these two strains seemed to have different system for accumulating Cd. So we detected changes in the soluble protein synthesis pattern by two-dimensional polyacrylamide gel electrophoresis for C. acidophila DVB 238 cells grown under different concentrations of Cd and Ni. Some of characteristic spots were analyzed by protein sequencer and MALDI-TOF Mass.

Journal

Details 詳細情報について

  • CRID
    1390001205627437952
  • NII Article ID
    130006987626
  • DOI
    10.14841/jspp.2005.0.409.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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