Cytochrome P450 <I>CYP710A</I> encodes the sterol C-22 desaturase in plants

DOI

Bibliographic Information

Other Title
  • 代謝プロファイリングによるステロール側鎖不飽和化酵素（CYP710A)の同定と機能解析

Abstract

Delta 22-unsaturated sterols, containing a double bond at the C-22 position in the side chain, occur specifically in fungi and plants. In yeast, cytochrome P450 (CYP61) has been known as the sterol Δ22-desaturase in the ergosterol biosynthetic pathway. In this study, we performed a comprehensive sterol profiling to identify functional homologues of CYP61 in plants. Thus, we prepared a series of P450 transgenic lines of Arabidopsis and studied differential effects on the sterol profiles in planta. In the overexpression lines of either CYP710A1 from Arabidopsis or CYP710A11 from tomato, stigmasterol accumulated to 6- to 32-fold higher levels compared with wild type. The promoter::GUS reporter assays suggested strict tissue specific expression patterns of the Δ22-desaturase genes in Arabidopsis, implicating developmental stage specific involvement of the Δ22-sterols.

Journal

Details 詳細情報について

  • CRID
    1390001205627571712
  • NII Article ID
    130006987761
  • DOI
    10.14841/jspp.2006.0.462.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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