Molecular Cloning and Gene Expression of Phospholipase A<SUB>2</SUB> in Tobacco
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- Fujikawa Ritsuko
- Graduate School of Biosphere Science, Hiroshima Univ.
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- Fujikawa Yukichi
- Graduate School of Biosphere Science, Hiroshima Univ.
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- Iijima Noriaki
- Graduate School of Biosphere Science, Hiroshima Univ.
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- Esaka Muneharu
- Graduate School of Biosphere Science, Hiroshima Univ.
Bibliographic Information
- Other Title
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- タバコのホスホリパーゼA<SUB>2</SUB>のcDNAクローニングと遺伝子発現
Abstract
Phosholipase A2(PLA2) hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. In animals, secreted PLA2s(sPLA2) have been recognized as a large super family of distinct enzymes that play a central role in diverse cellular processes. In plants, little is known about the biological functions of sPLA2, although there are some reports of purification and cDNA cloning of sPLA2. To investigate biological function of sPLA2 in tobacco, Nicotiana tabacum, we attempted to isolate cDNAs for sPLA2 from tobacco. Initially, we performed RT-PCR with primes selected on highly conserved regions of sPLA cDNAs using total RNA extracted from tobacco flower. Next, we performed 5'- and 3'-RACE using gene-specific primers derived from the respective partial sequences. The cDNAs of sPLA2 from tobacco encoded 145 and 159 amino acids, respectively. The deduced amino acid sequences were shown to have a putative signal peptide. At present, we have studied gene expression of sPLA2 in tobacco.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2003 (0), 446-446, 2003
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205627696896
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- NII Article ID
- 130006987961
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed