Functional analysis of PEPC in <I>Lotus japonicus</I>

DOI

Bibliographic Information

Other Title
  • ミヤコグサ根粒PEPC酵素の機能解析

Abstract

Phosphoenolpyruvate carboxylase (PEPC) is an enzyme that fixes carbon dioxide from the atmosphere by generating the oxaloacetic acid from pyruvate in the mesophyll cell of the C4 plant. However, the PEPC activity in legume nodule is about 100 times higher than that in leaf in Lotus japonicus. In order to find the function of the PEPC in the nodule, we tried to make the two kinds of transgenic plants, Ppc overexpressed plant (Ljpc) and Ppc suppressed plants (aLjpc), by Agrobacterium mediated transformation methods. In 21 DAI nodule, the level of Ljppc mRNA in the aLjpc plants had decreased compared with that in the non-transformant. However the level of Ljppc mRNA in the Ljpc plant was not so different from that in non-transformant. The PEPC activities in aLjpc plants were 8-10 times lower than that in non-transformant. The weights of 100 seeds of aLjpc plants were decreased compared to non-transformant

Journal

Details 詳細情報について

  • CRID
    1390001205628286720
  • NII Article ID
    130006988602
  • DOI
    10.14841/jspp.2004.0.568.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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