Metabolic Profiling Analysis Using FT-ICR MS

DOI

Bibliographic Information

Other Title
  • FT-ICR MS分析によるメタボリックプロファイリング解析～問題点と応用～

Abstract

We have developed a metabolic profiling experimental scheme based on a direct-infusion method on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS). The metabolic profiling scheme was designed for metabolome classification and marker metabolite identification, correcting considerable analytical errors intrinsically associated with the high-resolution analyses with FT-ICR MS under unpredictable ion suppression effects in the analytical cell. The scheme consists of 1) optimization of the analysis conditions for reproducible data correction among each MS scan, 2) development of a data analysis tool (Dmass) for automatic mass-error correction, 3) multivariate analyses for metabolome clarification among different samples, 4) identification of marker metabolite candidates through a database (KNApSAcK) search using accurate mass values, and 5) structural analyses for the candidate compounds by MSMS technique. We discuss the application of our experimental scheme for an integrated data analysis of metabolomics and transcriptomics for Arabidopsis suspension cells under different culture conditions.

Journal

Details 詳細情報について

  • CRID
    1390001205628465536
  • NII Article ID
    130006988802
  • DOI
    10.14841/jspp.2006.0.001.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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