Structural Studies of Rice Phytochrome B PAS1 Domain Using Multi-Dimensional NMR
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- Kobayashi Toshitatsu
- Graduate School of Biological Science, Nara Institute of Science and Technology
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- Mishima Masaki
- Graduate School of Biological Science, Nara Institute of Science and Technology
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- Tabata Ryo
- Graduate School of Biological Science, Nara Institute of Science and Technology
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- Akagi Kayo
- National Institute of Agrobiological Sciences
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- Sakai Nobuya
- National Institute of Agrobiological Sciences
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- Katoh Etsuko
- National Institute of Agrobiological Sciences
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- Takano Makoto
- National Institute of Agrobiological Sciences
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- Yamazaki Toshimasa
- National Institute of Agrobiological Sciences
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- Kojima Chojiro
- Graduate School of Biological Science, Nara Institute of Science and Technology
Bibliographic Information
- Other Title
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- イネ フィトクロムB PAS1ドメインの構造生物学的研究
Description
Phytochrom B (phyB) is a dimeric chromoprotein that detects the quantity, quality, and duration of red or far-red light throughout the entire life cycle of plants. Upon absorption of red light, phyB translocates from the cytoplasm to nucleus, and regulates gene expression through interaction with transcription factors such as basic-helix-loop-helix proteins. The PAS domain within the phyB C-terminal domain contains determinants necessary for nuclear translocation and signal transduction. Here we showed the solution structure of PAS1 domain, one of two PAS domains, determined by multidimensional NMR spectroscopy. The structure of a loss of function missense mutant was partially denatured. Our structural and biochemical data revealed that the N-terminal region of the PAS1 domain is necessary to form homodimer, and the PAS1 domain does not interact with the other PAS domain, PAS2.
Journal
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- Plant and Cell Physiology Supplement
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Plant and Cell Physiology Supplement 2005 (0), 569-569, 2005
The Japanese Society of Plant Physiologists
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Details 詳細情報について
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- CRID
- 1390001205628542592
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- NII Article ID
- 130006988909
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed